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This detailed volume explores the continuing techniques of§studying RNA-protein complexes and interactions as research in these areas§expand. After an introductory chapter,§the book continues with ways to purify RNA-protein complexes assembled in cells§or in isolated cellular extracts, methods for measuring various biochemical§activities of RNA-interacting proteins or ribonucleoproteins, biochemical§methods for measuring direct RNA-protein contact, as well as various new or§innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series,§chapters contain brief introductions to their respective topics, lists of the§necessary materials and reagents, step-by-step, readily reproducible laboratory§protocols, and tips on troubleshooting and avoiding known pitfalls.§§Authoritative and up-to-date, RNA-Protein§Complexes and Interactions: Methods and Protocols provides a set of useful§protocols, both basic and advanced, designed to inspire researchers working§with RNA and RNA-interacting proteins.
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