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Rapid Cell Magnetisation Using Cationised Magnetoferritin

Sara Correia Carreira

Language

English

Book Hardback

Libristo code: 16305045

Publishers Springer International Publishing AG, July 2017

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interd... Full description

Libristo code: 16305045

263 b

107.29 € VAT included

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Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.