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Molecular Studies on Preproinsulin Gene

Name: Molecular Studies on Preproinsulin Gene
Brand: LAP Lambert Academic Publishing
SKU: 15815291
Price: 31.7 EUR
Availability: InStock
Author: Sarah Sabir
ISBN: 9783330029064

Sarah Sabir

Language

English

Book Paperback

Libristo code: 15815291

Publishers LAP Lambert Academic Publishing, November 2016

Insulin plays an important role in maintaining the blood glucose level of the body. The beta-cells o... Full description

Libristo code: 15815291

78 b

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Insulin plays an important role in maintaining the blood glucose level of the body. The beta-cells of pancreas produce insulin in form of precursor that is preproinsulin. The gene of preproinsulin provides an interesting system for addressing question related to molecular evolution. Recombinant DNA technology has made it possible to isolate and sequence the chromosomal genes coding for unique protein products. Although preproinsulin of various organism has been isolated and cloned, but there is no report from buffalo (Bubalus bubalis) that is our major livestock. The genomic DNA of buffalo was isolated using Laura-Lee-Boodram method. The part of preproinsulin gene (596bp and 520bp) using BPPI-UPS and bpiful_F as forward and BC1-C as reverse primer was amplified. Cloning of amplified fragments of gene were performed in pCR 2.1 vector. Positive clones were screened on the basis of blue white selection. The band obtained on 596bp and 520bp after colony PCR confirmed the successful cloning of preproinsulin gene in pCR 2.1 vector.